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"G-Quadruplex DNA Methods and Protocols" ed. by Peter Baumann PDF eBook €1 buy download


"G-Quadruplex DNA: Methods and Protocols" ed. by Peter Baumann
Methods in Molecular Biology, Volume 608. Springer Protocols
Springer Science+Business Media | 2010 | ISBN: 1588299503 9781588299505 9781597453639 | 278 pages | PDF | 10 MB

In this book experts in the field present a collection of detailed techniques for studying G-quartet formation, dynamics, and molecular recognition.

All chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.
This book will be a useful resource for those familiar with G-quartets as well as an easy entry point for those researchers from diverse fields who are just developing an interest in the exciting implications of G-quadruplex DNA.

Includes coverage of a wide range of methodologies to study G-quadruplex structures
Provides detailed protocols which ensure successful re-recreation of experimental procedures
Supplies step-by-step instructions in order to enable researchers to set up their own high throughput screens for G-quadruplex ligands
Explores computational approaches for the detection of G-quadruplex-forming structures
Examines state-of-the-art physical, chemical, and biological methods
Contains tricks of the trade and valuable troubleshooting advice


Contents
Preface
Acknowledgements
Contributors
1 G-Quadruplexes: From Guanine Gels to Chemotherapeutics
2 Molecular Modeling and Simulation of G-Quadruplexes and Quadruplex-Ligand Complexes
3 Computational Approaches to the Detection and Analysis of Sequences with Intramolecular G-Quadruplex Forming Potential
4 Preparation of G-Quartet Structures and Detection by Native Gel Electrophoresis
5 Biochemical Techniques for the Characterization of G-Quadruplex Structures: EMSA, DMS Footprinting, and DNA Polymerase Stop Assay
6 Real-Time Observation of G-Guadruplex Dynamics Using Single-Molecule FRET Microscopy
7 Sedimentation Velocity Ultracentrifugation Analysis for Hydrodynamic Characterization of G-Quadruplex Structures
8 2-Aminopurine as a Probe for Guadruplex Loop Structures
9 Assessing DNA Structures with 1251 Radioprobing
10 Monitoring the Temperature Unfolding of G-Guadruplexes by UV and Circular Dichroism Spectroscopies and Calorimetry Techniques
11 Probing Telomeric G-Guadruplex DNA Structures in Cells with In Vitro Generated Single-Chain Antibody Fragments
12 Detection of G-Guadruplexes in Cells and Investigation of G-Quadruplex Structure of d(T2AG3)4 in K+ Solution by a Carbazole Derivative: BMVC
13 Isolation of G-Quadruplex DNA Using NMM-Sepharose Affinity Chromatography
14 Quantifying Interactions Between G-Quadruplex DNA and Transition-Metal Complexes
15 G4-FID: A Fluorescent DNA Probe Displacement Assay for Rapid Evaluation of Quadruplex Ligands
Index
with TOC BookMarkLinks




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"G-Quadruplex DNA Methods and Protocols" ed. by Peter Baumann PDF eBook
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